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Glycyrrhetinic acid (GA) and its precursor, 11-oxo-β-amyrin, are typical triterpenoids found in the roots of licorice, a traditional Chinese medicinal herb that exhibits diverse functions and physiological effects. In this study, we developed a novel and highly efficient pathway for the synthesis of GA and 11-oxo-β-amyrin in Saccharomyces cerevisiae by introducing efficient cytochrome P450s (CYP450s:...
•The Arabidopsis genome possesses two confirmed Cytochrome P450 Reductase (CPR) genes, ATR1 and ATR2, together with a third putative homologue, ATR3, which annotation is questionable.
•Phylogenetic analysis classified ATR3 as a CPR‐like protein sharing homologies with the animal cytosolic dual flavin reductases, NR1 and Fre‐1, distinct from the microsomal CPRs, ATR1 and ATR2. Like NR1 and Fre‐1, ATR3...
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